Phytochemical Profile and Antibacterial Activity Test of Medicine Plants in Southeast Sulawesi Against Salmonella Typhi YCTC as An Alternative Addition to Substitution Ingredients for Halal Products
DOI:
https://doi.org/10.24090/mabsya.v5i1.8055Keywords:
Phytochemical, Antibacterial, Salmonella typhi YCTC, S. crispus, A. bilimbi, C. sappan, Alternative Ingredients, Halal ProductAbstract
Phytochemical profile and antibacterial activity test performed on several medicinal plants methanol extracts of plants Keji beling (Strobilanthes crispus BI), Starfruit (Averrhoa bilimbi Linn.), Kangkung (Ipomoea aquatica), Secang (Caesalpinia sappan Linn.) and Jathropa (Jatropha curcas Linn.) existing in the Southeast Sulawesi. Phytochemical screening carried out by TLC method to identify classes of secondary metabolic compounds like alkaloids, flavonoids, tannins, saponins and triterpenoids, while the antibacterial activity test against the bacteria Salmonella typhi YCTC using the agar diffusion method with chloramphenicol as a positive control. The results obtained show that the extract of the roots and leaves of plants of S. crispus yield a diameter of 9 mm and 5 mm clear zone, the extract of the leaves of plants A. bilimbi yield a diameter of 9 mm clear zone, while the extract of the trunks and flowers of C. sappan. plants produce a clear zone diameter of 3,5 mm and 6 mm against the bacteria. extracts from plant parts that have activity against salmonella bacteria can then be further investigated to obtain compounds that have potential as antibacterial and can be used as alternatives as herbal medicinal ingredients in halal products.References
Adyana, I, K., Yulinah. E., Sigit J. I. K Fisheri N., dan Insanu M., 2004, Efek Ekstrak Daun Jambu Biji Daging Buah Putih dan Jambu Biji Daging Buah Merah sebagai Antidiare, Acta Pharmaceutica Indonesia, (1).
Anonim, 1986, Sediaan Galenik, Departemen Kesehatan Republik Indonesia, Jakarta.
Awoyinka, O.A., Balogun, I.O., dan Ogunnowo, A.A.. 2007. Phytochemical Screening and In Vitro Bioactivity Cnidoscolus aconitifolius (Euphorbiaceae). Journal of Medicinal Plants Research. 1 (3).
Cowan, M., 1999, Plant Product as Antimicrobial Agent, Clinical Microbiology Reviews, 12 (4) DOI: https://doi.org/10.1128/CMR.12.4.564
Dubale, S., Dereje, K., Ahmed, Z., Negera, A., Sultan, S., 2023, Phytochemical Screening and Antimicrobial Activity Evaluation of Selected Medicinal Plants in Ethiopia, Journal of Experimental Pharmacology, 15 DOI: https://doi.org/10.2147/JEP.S379805
Dwidjoseputro, D., 2010, Dasar-Dasar Mikrobiologi, Djambatan, Jakarta.
Ernilasari, Kamalliansyah, W., Fitmawati, Dewi, I, R., Zumaidar, Saudah, Rayhannisa, 2021., Antibacterial activity of leaves, flowers, and fruits extract of Etlingera elatiorfrom Nagan Raya District, Indonesia against Escherichia coliand Staphylococcus aureus., Biodiversitas, 22 (10) DOI: https://doi.org/10.13057/biodiv/d221039
Harborne, J, B., 2003, Metode Fitokimia : Penuntun Cara Modern Menganalisa Tumbuhan. Edisi II, Institut Teknologi Bandung, Bandung.
Hariana, A.. 2006. Tumbuhan Obat dan Khasiatnya Seri Pertama. Penerbit Penebar Swadaya : Jakarta.
Irmalia, W.R.. 2011. Daya Antibakteri Ekstrak Biji Coklat (Theobroma cacao) Varietas Farastero terhadap Streptococus mutans. Skripsi. Fakultas Kedokteran Gigi, Universitas Airlangga.
Kemenkes, 2006, Pedoman Pengendalian Demam Tifoid, Kementerian Kesehatan Republik Indonesia, Jakarta.
Khunaifi, M., 2010, Uji Aktivitas Antibakteri Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa, Skripsi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Maulana Malik Ibrahim, Malang.
Kristianingsih, 2005, Isolasi dan Identifikasi Senyawa Triterpenoid dari Akar Tanaman Kedondong Laut (Polyscias fruticosa), Skripsi, Universitas Brawijaya, Malang.
Kusnaeni, V., 2008, Isolasi dan Karakterisasi Senyawa Fraksi n-Heksana dari ekstrak kulit batang Angsret (Spathoda campanulata Beauv), Skripsi, Universitas Brawijaya, Malang.
Lutfillah, M., 2008, Karakterisasi Senyawa Alkaloid Hasil Isolasi dari Kulit Batang Angsret (Spathoda campanulata Beauv) Serta Uji Aktivitasnya Sebagai Antibakteri secara In Vitro, Skripsi, Universitas Brawijaya, Malang.
Mardjono, M., 1995, Farmakologi dan Terapi, Gaya Baru, Jakarta.
Megawati, 2020., Review: Phytochemical Screening, Secondary Metabolites and Biological Activities of Southeast Sulawesi Plants, Jurnal Akta Kimia Indonesia, 13 (2)
Pan, X., Chen, F., Wu, T., Tang, H., dan Zhao, Z. 2009. The Acid, Bile Tolerance and Antimicrobial Property of Lactobacillus acidophilus NIT. J. Food Control. 20 DOI: https://doi.org/10.1016/j.foodcont.2008.08.019
Pelczar, M J., dan Chan, E, C, S., 2005, Dasar-dasar Mikrobiologi Edisi 1, UI Press, Jakarta.
Pelczar, M J., dan Chan, E, C, S., 2007, Dasar-dasar Mikrobiologi Edisi 2, UI Press, Jakarta.
Pratiwi, S,T., 2008, Mikrobiologi Farmasi, Erlangga, Jakarta.
Rahmadani, S. S., 2013, Uji Daya Hambat Ekstrak Tanaman Komba – Komba (Chromolaena odorata) terhadap Pertumbuhan Staphylococcus aureus ATCC 25923, Streptococcus sp, Salmonella typhi YCTC, dan Escherechia coli ATCC 35218. Skripsi, Fakultas Kedokteran Universitas Halu Oleo, Kendari.
Ruslin dan Sahidin, I., 2008, Identifikasi dan Determinasi Tanaman Obat Tradisional Masyarakat Sulawesi Tenggara pada Arboretum Prof. Mahmud Hamundu, Majalah Farmasi Indonesia. 19 (2).
Supriyono, 2011, Demam Tifoid, Departemen Kesehatan Republik Indonesia, Jakarta.
Tanaka, J.C.A., C.C. da Silva, A.J.B. de Oliveira, C.V. Nakamura dan B.P. Dias Filho, 2006, Antibacterial activity of indole alkaloids from Aspidosperma ramiflorum, Braz J Med Biol Res, 39. DOI: https://doi.org/10.1590/S0100-879X2006000300009
Wahyono, H., 2010, Resistensi Antibiotik, Pidato pengukuhan guru besar Mikrobiologi FK UNDIP, Semarang.
Wardhani, K, L., dan Nanik, S., 2012, Uji Aktivitas Antibakteri Ekstrak Etil Asetat Daun Binahong (Anredera scandens (L.) Moq.) terhadap Shigella flexneri Beserta Profil Kromatografi Lapis Tipis. Jurnal Ilmiah Kefarmasian 2 (1) DOI: https://doi.org/10.12928/pharmaciana.v2i1.636
Widowati, R., Sri, H., Ahmad, R, A, F., 2021, Phytochemical Screening and Antibacterial Activities of Senggani (Melastoma malabathricumL.) Ethanolic Extract Leaves, Jurnal Ilmu Pertanian Indonesia (JIPI), 26 (4) DOI: https://doi.org/10.18343/jipi.26.4.562
Zega, T, S., Putri, M, P., Rahmayani, S., Givinda, S., Saronom, S., 2021, Antibacterial activity test of Simargaolgaol(Aglaonema modestumSchott ex Engl) leavesextract against Escherichia coliand Salmonella typhibacteria, Jurnal Pendidikan Kimia, 13 (2) DOI: https://doi.org/10.24114/jpkim.v13i2.26989
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